- Natural product: Green tea (Camellia sinensis)
- Associated study: Inhibitory Effects of Green Tea and (-)-Epigallocatechin Gallate on Transport by OATP1B1, OATP1B3, OCT1, OCT2, MATE1, MATE2-K and P-Glycoprotein
In Vitro Transporter Inhibition Experiment
Inhibition of MATE2-K by Green Tea
Inhibition was detected. Cutoff used — >20% inhibition vs. control
metformin 1503297
- MATE2-K (SLC47A2)
Transfected / siRNA knock-out / injected cells HEK293 transfected cells
Results
Percent inhibition in MATE2-K cells was estimated from Fig. 1. The IC50 value for inhibition of metformin by green tea (0.1-30% v/v) could not be calculated.
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
Sodium butyrate (10 mM) was added 24 h prior to uptake experiments to nonspecifically induce transporter expression.
5.0 x 10^5 cells/well
12-well poly-D-lysine coated plates
72 h
10 min
37 deg C
8
10 µM
30% v/v
Healthya green tea (Kao Corp., Tokyo, Japan)