- Natural product: Goldenseal (Hydrastis canadensis)
- Associated study: Poor permeability and absorption affect the activity of four alkaloids from Coptis
In Vitro Transporter Kinetics Experiment
Efflux of Berberine through P-gp
No Transport Activity was detected.
Cutoff used —
Papp >1.5x10-6 cm·s-1 is considered to be highly penetrative; therefore, the Papp of the four alkaloids assessed in the present
Papp >1.5x10-6 cm·s-1 is considered to be highly penetrative; therefore, the Papp of the four alkaloids assessed in the present
berberine 19012197
- P-gp (ABCB1)
Cell system Caco-2 cells
Results
Above results for 10 μmol
Papp A-B
20 μmol: 3.53
30 μmol: 2.85
60 μmol: 2.98
100 μmol: 2.68
Papp B-A
20 μmol: 31.30
30 μmol: 20.81
60 μmol: 25.30
100 μmol: 25.90
Ratio PappB-A / PappA-B
20 μmol: 8.87
30 μmol: 7.30
60 μmol: 8.49
100 μmol: 9.66
Table IV
| Sample | Compound measured | Value | Measurement | Study sequence | Additional information | N replicates |
|---|
Experimental Conditions
5x10^4/cm^2
12-well plate
Transwell
21
60 min
37 degrees C
7.4
10, 20, 30, 60, 100 μmol*mL^(-1)